The Acute and Subchronic Toxicity Evaluation of methanol leaf Extracts Of Bombaxbuonopozense

Abstract

Natural products serve as the foundation for conventional medicine and are significant sources of lead compounds for medication research. One of the underutilized plants in West Africa is Bombax buonopozense. West African populations use the majority of its parts for nutritional and therapeutic purposes.  The research was aimed at evaluation of the Acute and subchronic oral toxicity of   Bombaxbuonopozense leaves extract in rats.In the acute toxicity study, female rats received oral doses of 2000 mg/kg of Bombaxbuonopozense extract (n = 5/group). Abnormal behavior, toxic symptoms, weight changes, and mortality were monitored for 14 consecutive days to evaluate acute toxicity. For the sub-chronic toxicity study, the extract was orally administered to Wistar rats at doses of 800 and 1600 mg/kg (n = 5/group) daily for 28 days. Daily observations were made on the general behavior and body weight of the rats. Biochemical and hematological analyses were performed at the end of the treatment period. Following acute oral administration, the LD50 of Bombaxbuonopozens leaves extract was found to be greater than 2000 mg/kg in the evaluation of acute oral toxicity. Administration of Bombaxbuonopozense extract to rats did showed significantly increase in platelate in the treated group (362.00±23.67*) when compared to the control group (451.67±30.77) at (p>0.05). The liver enzymes, An increase (P>0.05) was seen in ALP concentration on the treated group (216.59±44.29*) In the subchronic oral toxicity assessment at doses of 800 and 1600 mg/kg/day for 28 days, no significant changes were observed in food consumption, body weight gain, organ weights, and biochemical parameters. Treatment of rats with Bombaxbuonopozense extract showed a significant increase in ALP on the group treated with 1600 mg/kg (216.59±44.29*) compared to the control (98.70±13.32) and significant increase in LDL for the treated groups 800 mg/kg (70.76±8.19*) and 1600 mg/kg (71.40±13.17*) were observed when compared with.The current research demonstrated that administration of  Bombaxbuonopozense extract orally for 28 days, at doses of up to 1600 mg/kg, did not cause any toxic effects. Based on the acute toxicity study, the estimated median lethal dose (LD50) of the extract was more than 2000 mg/kg.