Identification of a Novel ABC-2 Family Transporter Protein in Bacillus megaterium DSM319 Genome using Bioinformatics

Abstract

Bacillus megaterium is a gram positive rod shaped bacterium that is greatly responsible for the formation of spores aerobically and found throughout the world. The size of respective bacterium genome is 5.48 Mb and contains about 5,858 exons.  It has two strains named as QMB1551 and DSM319 having GC amount 38%. Regardless, a large number of the genes do not have any anticipated function, being depicted as hypothetical proteins. It is one of the most significant industrial bacterium that is employed for the generation of various useful products. It has played a promising role in the field of biotechnology as a production of genetically engineered proteins. Bioinformatics is one of the most advanced field that employed various productive tools in order to analyze the sequence, structure and function of proteins. In order to predict the function of a hypothetical protein of B. megaterium DSM319, BLAST was utilized for sequence comparison of hypothetical proteins of the bacterium dataset against the subject sequence of dataset of biotechnological important proteins. The best hit obtained from BLASTp results for sequence similarity was ABC-2 family transporter proteins having known functions. With that about 80% of similarity a daunorubicin ABC transporter permease (Anaerobacillus alkaliacustris) having query cover 100% and e-value 1 e-153 is obtained. The permease enzyme facilitates the ABC transporter to carry out export of daunorubicin that is produced by Bacillus spp. as a secondary metabolite. From the current study, it is predicted that B. megaterium having a protein known as daunorubicin ABC transporter permease. Moreover, this bacterium might be able to produce daunorubicin as a secondary metabolite. Daunorubicin has a great significance in medical sciences especially in anticancer therapy.